Isolation, purification, and partial characterization of formate dehydrogenase from soybean seed.
نویسندگان
چکیده
Soybean (Glycine soja var Beeson) formate dehydrogenase has been isolated, purified, and partially characterized by affinity chromatography. The enzyme is a dimer having a total molecular weight of 100,000 and a subunit weight of 47,000. It has activity over a broad pH range, is stable for months at 4 degrees C, and has K(m) values of 0.6 millimolar and 5.7 micromolar for formate and NAD, respectively.
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عنوان ژورنال:
- Plant physiology
دوره 73 3 شماره
صفحات -
تاریخ انتشار 1983